部分地区奶牛腹泻粪便样中诺如病毒的检测和演化分析

doi:10.11843/j.issn.0366-6964.2019.05.015

畜牧兽医学报 ›› 2019, Vol. 50 ›› Issue (5): 1048-1055.doi: 10.11843/j.issn.0366-6964.2019.05.015

部分地区奶牛腹泻粪便样中诺如病毒的检测和演化分析

王玥琳¹, 郭紫晶¹, 岳华^1,2, 汤承^1,2*

1. 西南民族大学生命与技术学院, 成都 610041;
2. 青藏高原动物遗传资源保护与利用教育部重点实验室, 成都 610041

收稿日期:2018-07-17 出版日期:2019-05-23 发布日期:2019-05-23
通讯作者: 汤承,主要从事动物病原微生物研究,Tel:028-85528276,E-mail:tangcheng101@163.com
作者简介:王玥琳(1995-),女,布依族,贵州荔波人,硕士生,主要从事动物病原微生物学研究,E-mail:905113619@qq.com
基金资助:
“十三五”国家重点研发计划课题（2016YFD0500907）；国家民委“青藏高原动物疫病防控创新团队”（13TD0057）；西南民族大学研究生“创新型科研项目”（CX2018SZ28）

Detection and Evolutionary Analysis of Bovine Noroviruses in Diarrheic Fecals of Dairy Calves in Some Areas

WANG Yuelin¹, GUO Zijing¹, YUE Hua^1,2, TANG Cheng^1,2*

1. College of Life Science and Technology, Southwest Minzu University, Chengdu 610041, China;
2. Key Laboratory of Qinghai-Tibetan Plateau Animal Genetic Resource Reservati on and Utilization, Chengdu 610041, China

Received:2018-07-17 Online:2019-05-23 Published:2019-05-23

摘要/Abstract

摘要：

牛诺如病毒（bovine norovirus，BNoV）是一种引起犊牛腹泻的病原，目前已在19个国家检出。本研究旨在调查该病毒在国内部分地区的流行情况及分子特征。采用RT-PCR方法检测了5个省12个奶牛场的93份腹泻粪便样本和54份健康粪便样本。结果显示：腹泻样本中BNoV检出率为25.81%，极显著高于健康粪便样本中9.26%的检出率（P<0.01），证明该病毒与犊牛腹泻具有相关性；基于29个RdRp序列的遗传进化分析显示，5份样本为GⅢ.1型，24份样本为GⅢ.2型。对获得的18个VP1和13个VP2基因片段的遗传进化分析发现，中国的BNoV毒株具有独特的演化趋势。本研究证明BNoV已在国内的奶牛中广泛流行，是国内犊牛腹泻的新发病原，且国内的BNoV毒株具有独特的演化趋势，为犊牛腹泻的防控提供了重要参考。

Abstract:

Bovine norovirus (BNoV) is a pathogen causing calf diarrhea and has been detected in 19 countries. The aims of this study were to investigate the prevalence and molecular characteristics of the virus in several regions of China. In this study, a total of 93 diarrheic fecal samples and 54 healthy fecal samples collected from 12 farms in 5 provinces were detected by RT-PCR. The results showed that the detection rate of BNoV in diarrhea samples (25.81%) was significantly higher than that of the healthy samples (9.26%) (P<0.01), which proved that the virus was associated with calf diarrhea. Phylogenetic analysis based on 29 RdRp sequences (partial polymerase sequences) showed that 5 strains clustered into GⅢ.1 and the remaining 24 strains clustered into GⅢ.2. Phylogenetic analysis based on 18 partial VP1 and 13 VP2 sequences showed that BNoV strains in China present a unique evolutionary. These results indicated that, as an emerging pathogen with unique evolutionary characteristics in China, BNoV has been widely circulated among dairy calves, providing an important reference for the prevention and control of calf diarrhea in China.

中图分类号:

S852.659.6

王玥琳, 郭紫晶, 岳华, 汤承. 部分地区奶牛腹泻粪便样中诺如病毒的检测和演化分析[J]. 畜牧兽医学报, 2019, 50(5): 1048-1055.

WANG Yuelin, GUO Zijing, YUE Hua, TANG Cheng. Detection and Evolutionary Analysis of Bovine Noroviruses in Diarrheic Fecals of Dairy Calves in Some Areas[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(5): 1048-1055.

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部分地区奶牛腹泻粪便样中诺如病毒的检测和演化分析

Detection and Evolutionary Analysis of Bovine Noroviruses in Diarrheic Fecals of Dairy Calves in Some Areas

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